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KMID : 0861020030180020059
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Korea Journal of Herbology
2003 Volume.18 No. 2 p.59 ~ p.70
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The Antioxidant Effect, cell viability and the effect to the gene expression using cDNA microarray assay of Opae-san
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Abstract
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Objectives : This study was undertaken to determine the effect of Opae-sun on antioxidant effect, cell viability and the expression of genes in human stomach cancer cells.
Methods : In order to analyze the Antioxidant Effect, cell viability and the effect to the gene expression of Opae-sun, human stomach cancer cells were stimulated by Opae-san using cDNA microarray chip. After that, abstracted mRNA went through in situ hybridization and it demonstrated the effect to the gene expression.
Results :
1. Scavenging effect of Opae-sun on DPPH radical treated with the DPPH radical did not demonstrated remarkably increased antioxidant effect in Opae-san treatment group.
2. MTT assay performed that more differentially increased cell viability in l00㎛/㎖ and l㎛/㎖ Opae-san treatment group than contrast.
3. 47 gene significantly increased by Opae-san treatment.
4. 391 gene significantly decreased genes by Opae-sun treatment.
Conclusions : Collectively, these results can be used as basic data for development of new anticancer drugs or treatment.
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KEYWORD
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